Under the conditions of sds - page , it depolymerized into only one subunit of 28 kd , which reveal that the subunit of purified ferritin of pea is undivided 4 . preparation of antiserum of pea ferritin the purified pea ferritin was mixed with freund ' s complete adjuvant or freund ' s incomplete adjuvant , and then administered through palmula , back , abdominal cavity , vena auricularis and other routes for rabbits . seven weeks later , the litre of antiserum reached 1 i 32 4 、免疫家兔制备豌豆铁蛋白抗血清民二功寻咬径b一仑一以纯化的豌豆铁蛋白作为抗原，并分别加入弗氏完全佐剂和弗氏不完全佐剂等兔疫家兔，经过足掌、背部皮下、腹腔和耳静脉等途径进行免疫， 7周后获得效价高达1 ： 32以上的豌豆铁蛋白抗血清。
Methods : in the first part , we survived and cultured m4g3 hybridoma in vitro . after two weeks of injecting hybridoma into mouse abdominal cavity , we collected abundant ascites . at the same time , 4 strains of breast cancer cells had been growing in vitro , digested and collected for immunocytochemistry and western blotting with m4g3 ascites 方法：第一部分：复苏m4g3杂交瘤细胞，体外培养，小鼠腹腔注射，进行单克隆抗体的大量制备；复苏t47d等四种乳腺癌细胞，体外培养，爬片，应用m4g3进行免疫细胞化学实验；四种细胞收集，应用m4g3进行western - blot染色。
Relax the belt after a meal , will enable pressing to drop in the abdominal cavity , in this way the function of support to alimentary canal will be weakened , and activity degree of digestive organs and load of the ligament will increase soon , it is apt to cause the gastroptosis , the disease of digestive system such as being uncomfortable of upper abdomen appears 饭后放松裤带，会使腹腔内压下降，这样对消化道的支持作用就会减弱，而消化器官的活动度和韧带的负荷量就要增加，容易引起胃下垂，出现上腹不适等消化系统疾病。
